Iba1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA12684

应用：

WB,IHC-P,IHC-F,IF,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 有库存
50μL ￥1250 有库存
100μL ￥2200 有库存

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

17 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

AIF 1; AIF1; AIF1 protein; IBa1; iba-1; Iba-1;IBa 1; allograft inflammatory factor 1; Allograft inflammatory factor 1 splice variant G; balloon angioplasty responsive transcription; BART 1; G1; G1 putative splice variant of allograft inflamatory factor 1; IBA 1; IBA1; interferon gamma responsive transcript; Ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule; IRT 1; IRT1; Microglia response factor; MRF1; Protein G1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:5000

IHC-P 1:1000

IHC-F 1:50

IF 1:50-1:100

FCM 1:50-1:200

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

AIF1 G1 IBA1

Protein Name：

Allograft inflammatory factor 1 (AIF-1)

Gene ID：

199 (Human)

11629 (Mouse)

29427 (Rat)

SwissPro：

P55008 (Human)

O70200 (Mouse)

P55009 (Rat)

Immunogen：

Synthetic peptide within N-terminal human Iba1.

Specificity:

Iba1 Monoclonal Antibody detects endogenous levels of Iba1 protein.

Product images
	Fig : Western blot analysis of Iba1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA12684, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: J774A.1 cell lysate
	Fig : Immunohistochemical analysis of paraffin-embedded rat-brain tissue with Rabbit anti-IBA1antibody (AWA12684) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12684 ) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded rat-cerebellum tissue with Rabbit anti-IBA1antibody (AWA12684) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12684 ) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti- IBA1(AWA12684) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12684) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.