SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10515

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 48 kDa

Observed MW: 62 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

P62; P62/SQSTM1; sequestosome 1; SQSTM1; Ubiquitin binding protein p62

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:5000

IHC-P 1:1000-1:2000

IF-C 1:100

IF-T 1:200-1:1000

FCM 1:1000

Immunogen

Information

Gene Name：

SQSTM1

Protein Name：

Sequestosome-1

Gene ID：

8878 (Human)

18412 (Mouse)

113894 (Rat)

SwissPro：

Q13501 (Human)

Q64337 (Mouse)

O08623 (Rat)

Subcellular Location：

Cytoplasm, Endoplasmic reticulum, Endosome, Lysosome, Nucleus.

Immunogen：

Synthetic peptide within Human SQSTM1/ p62. AA range: 400 to the C-terminus.

Specificity:

SQSTM1/p62 Monoclonal Antibody detects endogenous levels of SQSTM1/p62 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded rat-colon tissue with Rabbit anti-SQSTM1/P62 antibody ( AWA10515 ) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA10515 ) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded rat-spleen tissue with Rabbit anti-SQSTM1/P62 antibody ( AWA10515 ) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA10515 ) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of SQSTM1（P62) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody ( AWA10515, 1/1000) was used in TBST at room temperature for 90 minutes. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHZ-88 cell lysate Lane 2: RBL-2H3 cell lysate Lane 3: HeLa cell lysate Lane 4: NRF-49F cell lysate Lane 5: NIH3T3 cell lysate Lane 6: HEK293 cell lysate Lane 7: HepG2 cell lysate Lane 8: HCT116 cell lysate Predicted molecular weight: 48 kDa Observed molecular weight: 62 kDa
	Fig: Immunocytochemistry analysis of Hela cells labeling SQSTM1（p62）with Rabbit anti-SQSTM1（p62） antibody （AWA10515）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-SQSTM1（p62） antibody （AWA10515）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).