xCT/SLC7A11 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10085

应用：

WB,IP，IHC

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 有库存
50μL ¥1250 有库存
100μL ¥2200 有库存

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

55 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CCBR1; Cysteine/glutamate transporter; SLC7A11; solute carrier family 7; xCT

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IP 1-2μg/sample

IHC 1:100-1:400

Immunogen

Information

Gene Name：

SLC7A11

Protein Name：

Cystine/glutamate transporter

Gene ID：

23657 (Human)

26570 (Mouse)

310392 (Rat)

SwissPro：

Q9UPY5 (Human)

Q9WTR6 (Mouse)

Immunogen：

Synthetic peptide within Human xCT. AA range:1-112.

Specificity:

xCT Monoclonal Antibody detects endogenous levels of xCT protein.

Product images
	Fig : Western blot analysis of xCT/SLC7A11 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10085, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEPG2 cell Lane 2:SW620 cell Lane 3: A549 cell Lane 4: HEPA1-6 cell Lane 5: AML-12 cell Lane 6: HCT116 cell Lane 7: MC-38 cell Lane 8: PC-12 cell Lane 9: NPK-49F cell Lane 10: RBL-2H3 cell Calculated molecular weight：55 kDa Observed molecular weight：55 kDa, 35-40 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-small intestine tissue with Rabbit anti-xCT-SLC7A11 (AWA10085) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10085) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-xCT-SLC7A11 (AWA10085) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10085) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.