SQSTM1/p62 Mouse Monoclonal Antibody
-
-
- 20μL
- ¥620
- 现货
-
- 50μL
- ¥1250
- 现货
-
- 100μL
- ¥2200
- 现货
Product Details
| Host Species: Mouse | Reactivity: Human,Mouse,Rat | Molecular Wt: 48 kDa | |
Clonality: Monoclonal | Isotype: IgG1 | |||
Other Names: p60; p62; A170; OSIL; PDB3; ZIP3; p62B | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:500-1:2000 IHC 1:200-1:1000 IF 1:100-1:1000 ELISA 1:10000 FCM 1:200-1:400
| |||
Immunogen Information | Gene Name: SQSTM1 | Protein Name: Sequestosome-1 | ||
Gene ID: 8878 (Human) 18412 (Mouse) 113894 (Rat) | SwissPro: Q13501 (Human) Q64337 (Mouse) O08623 (Rat)
| |||
Subcellular Location: Cytoplasm, Endoplasmic reticulum, Endosome, Lysosome, Nucleus. | ||||
Immunogen: Recombinant fragment of human SQSTM1. AA range: 232-356. | ||||
Specificity: SQSTM1/p62 Monoclonal Antibody detects endogenous levels of SQSTM1/p62 protein. | ||||
Product images | |
Fig : Immunohistochemical analysis of paraffin-embedded mouse-brain tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig: Fluorescence immunohistochemical analysis of mouse-colon tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig : Immunohistochemical analysis of paraffin-embedded mouse-colon tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig: Fluorescence immunohistochemical analysis of mouse-cortex tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig: Fluorescence immunohistochemical analysis of mouse-hippocampus tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig : Immunohistochemical analysis of paraffin-embedded mouse-kidney tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig : Immunohistochemical analysis of paraffin-embedded rat-brain tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig: Fluorescence immunohistochemical analysis of RAT - colon tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig : Immunohistochemical analysis of paraffin-embedded rat-colon tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig: Fluorescence immunohistochemical analysis of RAT -cortex tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig : Immunohistochemical analysis of paraffin-embedded rat-kidney tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig : Western blot analysis of SQSTM1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody ( AWA00001, 1/1000) was used in TBST at room temperature for 90 minutes. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHZ-88 cell lysate Lane 2: RBL-2H3 cell lysate Lane 3: NRF-49F cell lysate Lane 4: NIH3T3 cell lysate Lane 5: HEK293 cell lysate Lane 6: HepG2 cell lysate Lane 7: HCT116 cell lysate |
-
-
- 20μL
- ¥620
- 现货
-
- 50μL
- ¥1250
- 现货
-
- 100μL
- ¥2200
- 现货
-
相关产品
-
Cytokeratin 15 Recombinant Rabbit Monoclonal Antibody
HMGB1 Recombinant Rabbit Monoclonal Antibody
SQSTM1/p62 Mouse Monoclonal Antibody
Vimentin Mouse Monoclonal Antibody
xCT/SLC7A11 Recombinant Rabbit Monoclonal Antibody
SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody
Caspase-8 Recombinant Rabbit Monoclonal Antibody
Ki67 Recombinant Rabbit Monoclonal Antibody