Vimentin Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA01208

应用：

WB,IHC,ELISA

反应性：

Human,Monkey

来源：

Mouse

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Monkey

Molecular Wt:

54 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Other Names:

VIM; Vimentin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:200-1:1000

ELISA 1:10000

Immunogen Information

Gene Name：

VIM

Protein Name：

Vimentin

Gene ID：

7431 (Human)

SwissPro：

P08670 (Human)

Immunogen：

Recombinant fragment of Vimentin. AA range:1-490.

Specificity:

Vimentin Monoclonal Antibody detects endogenous levels of Vimentin protein.

Product images
	Fig : Western blot analysis of Vimentin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA01208, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK293 cell lysate Lane 2: COS7 cell lysate Lane 3: Hela cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: A549 cell lysate Lane 6: K562 cell lysate Lane 7: Jurkat cell lysate Lane 8: HUVEC cell lysate Lane 9: U87-MG cell lysate Lane 10: A2058 cell lysate Predicted molecular weight: 53.6KD Observed molecular weight: 57KD Exposure time: 7 seconds
	Fig : Western blot analysis of Vimentin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA01208, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: C2C12 cell lysate Predicted molecular weight: 53.6KD Observed molecular weight: 57KD Exposure time: 45 seconds