HLA-DR Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA12675

应用：

WB,IHC,IF,FC

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 有库存
50μL ￥1250 有库存
100μL ￥2200 有库存

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse, Rat

Molecular Wt:

29 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

DRB1;DRB4;HLA DR1B;HLA DR3B;HLA DRA;HLADR4B;HLADRA1;HLADRB;MHC II;MLRW;MGC117330

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1,000

IHC-P 1:50-1:200

IF 1:50-1:200

FC 1:50-1:100

Immunogen

Information

Gene Name：

HLA-DRA HLA-DRA1

Protein Name：

HLA class II histocompatibility antigen, DR alpha chain (MHC class II antigen DRA)

Gene ID：

3122 (Human)

SwissPro：

P01903 (Human)

Immunogen：

Synthetic peptide within Human HLA-DRA aa 159-195 / 254.

Specificity:

HLA-DR Monoclonal Antibody detects endogenous levels of HLA-DR protein.

Product images
	Fig: Immunocytochemistry analysis of Hela cells labeling HLA-DR with Rabbit anti-HLA-DR antibody （AWA12675）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-HLA-DR antibody （AWA12675）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Immunohistochemical analysis of paraffin-embedded rat-brain tissue with Rabbit anti-HLA-DR antibody ( AWA12675 ) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded rat-liver tissue with Rabbit anti-HLA-DR antibody ( AWA12675 ) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded rat-spleen tissue with Rabbit anti-HLA-DR antibody ( AWA12675 ) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of HLA-DR on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12675, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell Predicted band size: 29 kDa Observed band size: 35 kDa
	Fig : Western blot analysis of HLA-DR on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12675, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: GL261 cell Lane 2: EL-4-B5 cell Lane 3: NRK-49F cell Lane 4: Raji cell Exposure time: 3min Predicted band size: 29 kDa Observed band size: 36 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-HLA-DR (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-HLA-DR (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-HLA-DR (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-thymus tissue with Rabbit anti-HLA-DR (AWA12675) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12675) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.