Collagen III Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA00128

应用：

IHC,ELISA

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat

Molecular Wt:

139 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Other Names:

COL3A1; Collagen alpha-1(III) chain; collagen, type III; Collagen Type III; Collagen III; alpha 1; EDS4A; FLJ34534

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

IHC 1:200-1:400

ELISA 1:5000-1:20000

Immunogen

Information

Gene Name：

COL3A1

Protein Name：

Collagen alpha-1(III) chain

Gene ID：

1281 (Human)

12825 (Mouse)

84032 (Rat)

SwissPro：

P02461 (Human)

P08121 (Mouse)

P13941 (Rat)

Subcellular Location：

Secreted, extracellular space, extracellular matrix.

Immunogen：

Synthesized peptide derived from human Collagen Type III. AA range: 100-200.

Specificity:

Collagen III Monoclonal Antibody detects endogenous levels of Collagen III protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-myocardium tissue with Mouse anti-COL3A1 (AWA00128) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00128) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-muscle tissue with Mouse anti-COL3A1 (AWA00128) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00128) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-small intestine tissue with Mouse anti-COL3A1 (AWA00128) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00128) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded mouse-lung tissue with Mouse anti-COL3A1 antibody (AWA00128) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00128) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.