α-Tubulin Recombinant Rabbit Monoclonal Antibody [40F03]

货号：

AWA86004

应用：

WB,IHC-P,IF-C,IF-P,FCM,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
50μL ¥580 1-3个工作日
100μL ¥920 1-3个工作日
500μL ¥3800 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 50 kDa
Observed MW: 50 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.186mg/ml

Other Names:

TUBA1B; Tubulin alpha 1B chain; Tubulin alpha; Alpha-tubulin ubiquitous; ALS22; TUBA1; H2-ALPHA; TUBA4A; Tubulin alpha-4A chain; α-Tubulin; Alpha Tubulin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:5000-1:10000
IHC-P 1:200-1:1000
IF-C 1:200-1:1000
IF-P 1:200-1:1000
FCM 1:50-1:200
ELISA 1:10000

Immunogen
Information

Gene Name:

TUBA4A

Protein Name:

Tubulin alpha-4A chain

Gene ID:

7277 (Human)
22145 (Mouse)
316531 (Rat)

SwissPro:

P68366 (Human)
P68368 (Mouse)
Q5XIF6 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm, cytoskeleton.

Immunogen:

Human MBP (microtubule-binding protein).

Specificity:

α-Tubulin Monoclonal Antibody detects endogenous levels of α-Tubulin protein.

Product images
	Fig : Western blot analysis of α-Tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA86004, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-12 cell Lane 2: NIH3T3 cell Lane 3: K562 cell Lane 4: Hek293 cell Predicted molecular weight:50 kDa Observed molecular weight:50 kDa Exposure time: 7 seconds

	Fig : Western blot analysis of α-Tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA86004, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: A431 cell Lane 3: Jurkat cell Lane 4: PC12 cell Predicted molecular weight:50 kDa Observed molecular weight:50 kDa

	Fig: Immunocytochemistry analysis of HELA cells labeling α-Tubulin with Rabbit anti-α-Tubulin antibody (AWA86004) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-α-Tubulin antibody (AWA86004) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebrum tissue with Rabbit anti-α-tubulin antibody (AWA86004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA86004) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-cerebellum tissue with Mouse anti-α-tubulin antibody (AWA86004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA86004) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-blood vessel tissue with Rabbit anti-α-Tubulin (AWA86004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA86004) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-α-Tubulin (AWA86004) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA86004) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig：Flow cytometric analysis of HELA cells labeling α-tubulin. Overlay histogram showing HELA cells stained with α-tubulin (green line). The cell were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA86004, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005b) at 1/2000 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

	Fig: Fluorescence immunohistochemical analysis of Rat-cerebral cortex tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-α-Tubulin antibody (AWA86004) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA86004) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

引用文献 (1)

3 Biotech IF:2.9

Expression and prognosis of mPRα/PAQR7 in human cancers and validation in lung squamous cell carcinoma

Membrane progesterone receptor α (mPRα)/ Progestin and adipoQ receptor family member 7(PAQR7), has been insufficiently studied in pan-cancer. This study analyzed its expression, diagnostic value, and correlations with clinical data, tumor stemness, genomic instability, prognosis, interacting molecules, RNA methylation phenotypes, and immune cell infiltration, immune-related genes, and immune checkpoints via multiple databases. It also evaluated mPRα’s effects on LUSC cell malignancy using cell counting kit-8 (CCK-8), colony formation, transwell, and flow cytometry, detected its expression in lung squamous cell carcinoma (LUSC) tissue microarray by immunohistochemistry (IHC) staining, and analyzed its overall survival (OS) correlation in LUSC via Kaplan-Meier curves. Results showed PAQR7 up-regulated and down-regulated in some cancers. It had diagnostic value in some cancers and correlated with clinical data, tumor stemness, genomic instability, and prognosis in some tumors. PAQR7 interacted with various molecules, focusing on hormone-mediated signaling pathways in biological process (BP), steroid binding in molecular function (MF), and chemical carcinogenesis-receptor activation in Kyoto Encyclopedia of Genes and Genomes (KEGG). PAQR7 was related to tumor angiogenesis, differentiation, and stemness. PAQR7 expression further showed associations with immune cell infiltration, related immune genes, and immune checkpoints. More importantly, validation experiments demonstrated that mPRα was aberrantly overexpressed in LUSC cells, and its knockdown impaired the malignant characteristics of LUSCs. In addition, higher mPRα protein expression was associated with a poor OS in LUSC patients. In conclusion, mPRα/PAQR7 was abnormally expressed in various cancers, and its high expression was associated with malignant phenotypes and poor prognosis in LUSC.

pubTime 2025-11-22

Application

Specie

Human

Dilution

1:10000