β-Tubulin Recombinant Mouse Monoclonal Antibody [40A10]

货号：

AWA86003

应用：

WB,IHC-P,IF-C,IF-P,FCM,ELISA

反应性：

Human,Mouse,Rat,Monkey

来源：

Mouse

规格 价格 库存
50μL ¥580 1-3个工作日
100μL ¥920 1-3个工作日
500μL ¥3800 1-3个工作日

产品概述

Product Details	Host Species: Mouse	Reactivity: Human, Mouse, Rat, Monkey		Molecular Wt: Predicted MW: 50 kDa Observed MW: 50 kDa
	Clonality: Monoclonal	Isotype: IgG2a		Concentration: 1.113mg/ml
	Other Names: Beta 4 tubulin; Beta 5 tubulin; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; BetaTubulin; Beta Tubulin; β-Tubulin
	Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
	Purification: Affinity-chromatography
	Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Applications	WB 1:10000-1:100000 IHC-P 1:200-1:1000 IF-C 1:200-1:1000 IF-P 1:200-1:1000 FCM 1:20-1:500 ELISA 1:10000
Immunogen Information	Gene Name: TUBB		Protein Name: Tubulin beta chain
	Gene ID: 203068 (Human) 22154 (Mouse) 29214 (Rat)		SwissPro: P07437 (Human) P99024 (Mouse) P69897 (Rat)
Immunogen Information	Subcellular Location: Cytoplasm, cytoskeleton.
	Immunogen: Human MBP (microtubule-binding protein).
	Specificity: β-Tubulin Monoclonal Antibody detects endogenous levels of β-Tubulin protein.
RRID	AB_3752808

Product images
	Fig : Western blot analysis of β-Tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA86003, 1/10000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: HepA1-6 cell Lane 3: HSC-T6 cell Lane 4: Rat brain Lane 5: Hela cell Lane 6: HEK293 cell Lane 7: Jurkat cell Lane 8: Raw264.7 cell Lane 9: RBL-2H3 cell Lane 10: 3D4/21 cell Lane 11: SK-N-SH cell Predicted molecular weight:50 kDa Observed molecular weight:50 kDa

	Fig: Immunocytochemistry analysis of HELA cells labeling β-Tubulin with Mouse anti-β-Tubulin antibody (AWA86003) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-β-Tubulin antibody (AWA86003) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-cerebrum tissue with Mouse anti-β-tubulin antibody (AWA86003) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA86003) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig：Flow cytometric analysis of HELA cells labeling β-tubulin. Overlay histogram showing HELA cells stained with β-tubulin (green line). The cell were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA86003, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody (AWS0003a) at 1/2000 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Mouse anti-β-Tubulin antibody (AWA86003) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA86003) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-fallopian tube tissue with Mouse anti-β-Tubulin antibody (AWA86003) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA86003) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

引用文献 (1)

JOURNAL OF HAZARDOUS MATERIALS IF:11.3

Polystyrene and Polyvinyl Chloride Microplastics Exposure Induces Ocular Surface Inflammation by Causing Mitochondrial Damage and Lipid Metabolic Disruption

Microplastics (MPs), particularly polystyrene (PS) and polyvinyl chloride (PVC), are pervasive environmental pollutants implicated in ocular surface damage through poorly understood molecular mechanisms. Here, we elucidate the pathways underlying PS/PVC-induced ocular surface inflammation. In vitro , PS and PVC particles were internalized by human corneal epithelial cells – transformed (HCE-T) cells, inducing cytotoxicity and robust pro-inflammatory responses. In vivo , PS/PVC exposure elicited dry eye–like phenotypes in the murine ocular surface. Integrated transcriptomic and metabolomic analyses revealed that PS/PVC trigger inflammatory cascades via mitochondrial damage and disruption of lipid metabolism. Specifically, aberrant opening of the mitochondrial permeability transition pore (mPTP), leakage of mitochondrial DNA (mtDNA), and dysregulation of lipid metabolism–associated genes LIPG and GRB14 emerged as key drivers of inflammation in HCE-T cells. Concurrently, PS/PVC-induced energy stress enhanced lipid droplet–mitochondria tethering. Notably, corneal inflammation was markedly attenuated by treatment with the mitochondria-targeted antioxidant SkQ1 and an adiponectin receptor agonist. Collectively, these findings delineate a mitochondria- and lipid metabolism–mediated mechanism for PS/PVC-induced ocular surface inflammation, offering mechanistic insights and potential therapeutic strategies for mitigating microplastic-associated ocular toxicity.

pubTime 2025-12-30

Application

Specie

Mouse,Human

Dilution