β Ⅲ tubulin Recombinant Mouse Monoclonal Antibody [45E09]

货号：

AWA80019

应用：

WB,IHC-P,IF-C,IF-P,FCM

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
50μL ¥580 1-3个工作日
100μL ¥920 1-3个工作日
500μL ¥3800 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 50 kDa
Observed MW: 52 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1mg/ml

Other Names:

beta 3 tubulin; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; QccE-11995; QccE-15186; TBB3_HUMAN; Tubb 3; TUBB3; TUBB4; Tubulin beta 3; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:5000-1:100000
IHC-P 1:200-1:1000
IF-C 1:200-1:1000
IF-P 1:200-1:1000
FCM 1:50-1:200

Immunogen
Information

Gene Name:

TUBB3

Protein Name:

Tubulin beta-3 chain

Gene ID:

10 3 8 1 (Human)
22152 (Mouse)
246118 (Rat)

SwissPro:

Q13509 (Human)
Q9ERD7 (Mouse)
Q4QRB4 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm, cytoskeleton. Cell projection, growth cone. Cell projection, lamellipodium. Cell projection, filopodium.

Immunogen:

Synthetic peptide within human Tubulin beta-3 chain. AA range: 401-450.

Specificity:

β Ⅲ tubulin Monoclonal Antibody detects endogenous levels of β Ⅲ tubulin protein.

Product images
	Fig : Western blot analysis of β Ⅲ tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA80019, 1/25000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain Lane 2: HepG2 cell Lane 3: Rat brain Lane 4: U87-MG cell Lane 5: SH-SY5Y cell Lane 6: HT22 cell Lane 7: N-2A cell Lane 8: PANC-1 cell Predicted molecular weight:50 kDa Observed molecular weight:52 kDa Exposure time: 3seconds

	Fig: Immunocytochemistry analysis of HELA cells labeling β Ⅲ tubulin with Mouse anti-β Ⅲ tubulin antibody (AWA80019) at 1/200 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-β Ⅲ tubulin antibody (AWA04223) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Mouse anti-β Ⅲ tubulin antibody (AWA80019) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA04223) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Fluorescence immunohistochemical analysis of Rat-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Mouse anti-β Ⅲ tubulin antibody (AWA80019) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA04223) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Mouse anti-β Ⅲ tubulin antibody (AWA80019) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA04223) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebral cortex tissue with Rabbit anti-β Ⅲ tubulin antibody (AWA80019) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80019) at 1/500 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.