Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody [44B04]

货号：

AWA11936

应用：

WB,IHC-P,IF-C,IF-P

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
50μL ¥580 1-3个工作日
100μL ¥920 1-3个工作日
500μL ¥3800 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 113 kDa
Observed MW: 100/113 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.046mg/ml

Other Names:

ATPase Na+/K+ transporting alpha; adenosinetriphosphatase; AT1A1_HUMAN; ATP1A1; ATP1A4; ATP1AL2; ATP1B; ATP1B1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting alpha 4 polypeptide; ATPase Na+/K+ transporting beta 1 polypeptide; ATPase, Na+/K+ transporting, alpha polypeptide-like 2; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+), K(+)-ATPase; Na(+)/K(+) ATPase alpha-1 subunit; Na(+)/K(+) ATPase alpha-4 subunit; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase 1; Na+/K+ ATPase 4; Na+/K+ ATPase, alpha-D polypeptide; Na, K-ATPase beta-1 polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na, K-ATPase catalytic subunit alpha-A protein; Na, K-ATPase subunit alpha-C; polypeptide-like 2; Sodium pump 1; sodium pump 4; Sodium pump subunit alpha-1; sodium pump subunit alpha-4; sodium pump subunit beta-1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:10000
IHC-P 1:200-1:5000
IF-C 1:200-1:1000
IF-P 1:200-1:1000

Immunogen
Information

Gene Name:

ATP1A1 ATP1B1 ATP1A4

Protein Name:

Sodium/potassium-transporting ATPase subunit alpha-1 Sodium/potassium-transporting ATPase subunit beta-1 Sodium/potassium-transporting ATPase subunit alpha-4

Gene ID:

476/481/480 (Human)
11928/11931/27222 (Mouse)
24211/25650/2913 2 (Rat)

SwissPro:

P05023/P05026/Q13733 (Human)
Q8VDN2/P14094/Q9WV27 (Mouse)
P06685/P07340/Q64541 (Rat)

Immunogen
Information

Subcellular Location:

Cell membrane. Melanosome.

Immunogen:

Synthetic peptide within human ATP1A1. AA range: 39-83.

Specificity:

Sodium Potassium ATPase Monoclonal Antibody detects endogenous levels of Sodium Potassium ATPase protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of Rat-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Fluorescence immunohistochemical analysis of Rat-placenta tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig : Western blot analysis of Sodium Potassium ATPase on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11936, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC12 cell Lane 2: Mouse brain（unboiled） Lane 3: Rat brain（unboiled） Predicted molecular weight:113 kDa Observed molecular weight:113 kDa

	Fig : Western blot analysis of Sodium Potassium ATPase on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11936, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH3T3 cell Lane 2: PC-12 cell Lane 3: SH-SY5Y cell Lane 4: L929 cell Lane 5: Hela cell Predicted band size: 113 kDa Observed band size: 100 kDa Exposure time: 7 seconds

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-small intestine tissue with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Fluorescence immunohistochemical analysis of Rat-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Fluorescence immunohistochemical analysis of Mouse-duodenum tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Fluorescence immunohistochemical analysis of Rat-liver tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-small intestine tissue with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Fluorescence immunohistochemical analysis of Rat-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11936) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig：Flow cytometric analysis of HELA cells labeling Sodium Potassium ATPase Overlay histogram showing HELA cells stained with Sodium Potassium ATPase (green line). The cell were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA11936, 1:100) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005b) at 1/1500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

	Fig: Immunocytochemistry analysis of MCF-7 cells labeling Sodium Potassium ATPase with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Sodium Potassium ATPase antibody (AWA11936) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).