Lamin B1 Recombinant Rabbit Monoclonal Antibody [45B05]

货号：

AWA11310

应用：

WB,IHC-P,IF-P,mIHC

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
50μL ¥580 1-3个工作日
100μL ¥920 1-3个工作日
500μL ¥3800 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 66 kDa
Observed MW: 66,72 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.106mg/ml

Other Names:

LMNB1; LMN2; LMNB; Lamin-B1; lamin B1; ADLD; LMN; MCPH26; Lamin B1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-P 1:100-1:1000
mIHC 1:100-1:1000

Immunogen
Information

Gene Name:

LMNB1

Protein Name:

Lamin-B1

Gene ID:

4001 (Human)
16906 (Mouse)
116685 (Rat)

SwissPro:

P20700 (Human)
P14733 (Mouse)
P70615 (Rat)

Subcellular Location:

Nucleus lamina.

Immunogen:

Synthetic peptide within human Lamin B1. AA range: 511-560.

Specificity:

Lamin B1 Monoclonal Antibody detects endogenous levels of Lamin B1 protein.

Product images
	Fig: Multiplex immunohistochemistry analysis of Mouse-intestinal tissue (Formalin/PFA-fixed paraffin-embedded sections). Merged staining of anti-β III tubulin (AWA80027; green; TSA-520:AWI0688), anti-Lamin B1 (AWA11310; red; TSA-570: AWI0689), anti-Aquaporin 4 (AWA10201; white; TSA-690: AWI0691).Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. The section was incubated in three rounds of staining; in the order of β III tubulin (AWA80027) (1/800 dilution), anti-Lamin B1 (AWA11310) (1/200 dilution), anti-AQP4 (AWA10493) (1/200 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi.

	Fig : Western blot analysis of Lamin B1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11310, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A2780 cell Lane 2: Raw264.7 cell Lane 3: 4T1 cell Lane 4: PC12 cell Lane 5: Rat heart Predicted molecular weight:66 kDa Observed molecular weight:66-72 kDa

	Fig : Western blot analysis of Lamin B1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11310, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell Lane 2: 786-O cell Lane 3: Mouse heart Lane 4: Hela cell Lane 5: SH-SY5Y cell Lane 6: HEK293 cell Predicted molecular weight:66 kDa Observed molecular weight:66-72 kDa

	Fig: Multiplex immunohistochemistry analysis of Rat-intestinal tissue (Formalin/PFA-fixed paraffin-embedded sections). Merged staining of anti-Cytochrome C (AWA10373; green; TSA-520:AWI0688), anti-EPCAM (AWA13608; red; TSA-570: AWI0689), anti-Lamin B1 (AWA11310; white; TSA-690: AWI0691). Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. The section was incubated in three rounds of staining; in the order of Cytochrome C (AWA10373) (1/200 dilution), anti-EPCAM (AWA13608) (1/200 dilution), anti-Lamin B1 (AWA11310) (1/200 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi.

	Fig: Fluorescence immunohistochemical analysis of Rat-liver tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Lamin B1 antibody (AWA11310) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11310) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Spleen tissue with Rabbit anti-Lamin B1 antibody (AWA11310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Lung tissue with Rabbit anti-Lamin B1 antibody (AWA11310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Kidney tissue with Rabbit anti-Lamin B1 antibody (AWA11310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Colon tissue with Rabbit anti-Lamin B1 antibody (AWA11310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Brain tissue with Rabbit anti-Lamin B1 antibody (AWA11310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Heart Muscle tissue with Rabbit anti-Lamin B1 antibody (AWA11310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.