Vinculin Recombinant Mouse Monoclonal Antibody [46B12]

货号：

AWA00533

应用：

WB,IHC-P,IF-C,IF-P,IP

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
50μL ¥580 1-3个工作日
100μL ¥920 1-3个工作日
500μL ¥3800 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 124 kDa
Observed MW: 124 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

0.950mg/ml

Other Names:

VCL; Metavinculin; CMD1W; MVCL; MV; CMH15; HEL114; Vinculin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:10000-1:100000

IHC-P 1:200-1:1000

IF-C 1:100-1:1000

IF-P 1:200-1:1000

IP 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate.

Immunogen
Information

Gene Name:

VCL

Protein Name:

Vinculin

Gene ID:

7414 (Human)

22330 (Mouse)

305679 (Rat)

SwissPro:

P18206 (Human)

Q64727 (Mouse)

P8597 2 (Rat)

Immunogen
Information

Subcellular Location:

Cell membrane. Cell junction, adherens junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cell membrane, sarcolemma. Cell projection, podosome. Cytoplasm, perinuclear region.

Immunogen:

Synthetic peptide within human Vinculin. AA range: 1000-1060.

Specificity:

Vinculin Monoclonal Antibody detects endogenous levels of Vinculin protein.

Product images
	Fig : Western blot analysis of Vinculin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00533, 1/10000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: HaCaT cell Lane 3: A431 cell Lane 4: A549 cell Lane 5: PC12 cell Lane 6: HepA1-6 cell Lane 7: Mouse heart Predicted molecular weight:124 kDa Observed molecular weight:124 kDa

	Fig：Immunoprecipitation of Vinculin from Hela cells was performed using Vinculin Mouse mAb (AWA00533,1:250). Mouse IgG isotype control was used to precipitate the Control IgG sample. The IP sample was eluted with Glycine buffer. Western blot analysis of immunoprecipitates was conducted using Vinculin Rabbit mAb (AWA10165) at a dilution of 1:1000. Goat Anti-Rabbit IgG(H+L) - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-heart muscle tissue with Mouse anti-Vinculin antibody (AWA00533) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with EDTA(pH 9.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00533) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.