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Hanks平衡盐溶液(1×HBSS,无酚红)

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货号:AWC0158

价格:¥140

规格:500ml

  • 产品概述
  • Hanks平衡盐溶液(1×HBSS,酚红)

    产品简介:

    平衡盐溶液(Balanced Salt Solution,BSS)与细胞生长状态下的pH值、渗透压等环境状态一致,具有维持渗透压、控制酸碱平衡、供给细胞生存代谢所必需的能量和无机盐成分等作用,可满足体外实验中细胞生存并维持一定的代谢的基本需要。主要由无机离子组成,有时含有碳酸氢钠、葡萄糖、酚红等,如果有必要还可以加入HEPES以维持渗透压的平衡。BBS配方常有改动,Hank's BBS有不含钙镁或酚红的,也有含钙镁含酚红的等,Dulbecco's PBS有不含钙镁或含钙镁的等。HBSS、EBSS、PBS等都是与较弱的磷酸盐缓冲液相关的盐溶液。常见的平衡盐溶液有Eargle's液、Hank's液、磷酸盐缓冲溶液(PBS)等。Hanks平衡盐溶液是最常用的磷酸盐缓冲溶液之一,又称Hanks' Balanced Salt Solution、Hanks' BSS、HBSS,主要由氯化钠、氯化钾、磷酸盐、碳酸氢钠、葡萄糖等组成,含Ca2+、Mg2+ 、不含酚红,pH值一般为7.2~7.4。

    Hanks平衡盐溶液(1×,无酚红),含Ca2+、Mg2+,不含酚红,用重蒸超纯水配制,pH值7.4,经严格过滤除菌处理,内毒素含量低,可用于胰蛋白酶-EDTA细胞消化液等各种无或低钙镁细胞培养用液的配制以及组织和细胞的漂洗等,直接使用。

    操作步骤(仅供参考):

    1、 无需配制,直接使用。

    注意事项:

    1、 在进行细胞培养过程中细胞的洗涤时,应注意无菌操作,避免被微生物污染。

    2、 Hanks平衡盐溶液(1×,无酚红)由于含钙离子,易产生沉淀,出现该种情况后,一般置于温浴至沉淀溶解即可,如果产生较多沉淀应弃用。

    3、 该试剂经过滤除菌。

    4、 为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。

    5、 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

    产品组成
    名称货号规格storage
    Hanks平衡盐溶液(1×,无酚红)AWC0158-500ml500ml4℃

    注意:

    1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗。食品及化妆品等用途。请勿存放于普通住宅区。

    2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。

    3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。


    参考文献 (1)

    FREE RADICAL BIOLOGY AND MEDICINE IF:8.2

    Metabolic dysfunction-associated steatohepatitis (MASH) is a complex liver disease whose pathogenesis involving endoplasmic reticulum (ER) stress and ferroptosis. However, key regulatory genes remain poorly understood, hindering the development of effective therapeutic targets. This study aims to identify genes linked to ER stress and ferroptosis through bioinformatics and experimental validation, providing insights into MASH pathogenesis and potential therapeutic strategies. We first identified ER stress and ferroptosis as key processes in MASH through differential analysis and functional enrichment. This was subsequently validated in a high-fat diet (HFD)-induced MASH model in ApoE -/- mice, where ER stress and ferroptosis were confirmed to occur in the liver tissue of MASH mice. Additionally, daily intraperitoneal injection of the ferroptosis inhibitor ferrostatin-1 (Fer-1) alleviated MASH progression. In vitro, Fer-1 mitigated inflammation, lipid accumulation, and fibrosis in free fatty acid (FFA)-treated HepG2 cells. To identify key genes, we employed bioinformatics analysis and machine learning approaches, which led to the identification of cyclin dependent kinase inhibitor 1A ( CDKN1A) and early growth response 1 ( EGR1) as feature genes associated with MASH-related ER stress and ferroptosis. Increased expression of CDKN1A and decreased expression of EGR1 were observed in the liver tissue of MASH mice and FFA-treated HepG2 cells. Furthermore, in CDKN1A overexpression and EGR1 silencing cell models, treatment with the ER stress inhibitor 4-Phenylbutyric acid improved the ferroptosis. In summary, all results indicate that CDKN1A and EGR1 are key genes driving ER stress-induced ferroptosis in MASH. Our findings not only provide new evidence for the pathogenesis of MASH but also highlight novel therapeutic targets for intervention.

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