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GDF15 Rabbit Polyclonal Antibody

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货号:
AWA68343
应用:
WB,IHC-P,IF-C,IP,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human, Mouse, Rat

    Molecular Wt:

    34 kDa

     

    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml

     

    Other Names:

    Growth/differentiation factor 15; growth differentiation factor 15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; GDF15

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:5000

    IHC-P 1:100-1:500

    IF-C 1:100-1:500

    IP 0.5-4.0 ug for 0.5-3.0 mg of total protein lysate.

    ELISA 1:20000

    Immunogen
    Information

    Gene Name:

    GDF15

    Protein Name:

    Growth/differentiation factor 15

     

    Gene ID:

    9518 (Human)

    23886 (Mouse)

    29455 (Rat)

    SwissPro:

    Q99988 (Human)

    Q9Z0J7 (Mouse)

    Q9Z0J6 (Rat)

    Immunogen
    Information

    Subcellular Location:

    Secreted.

     

    Immunogen:

    Recombinant protein within human GDF15.

     

    Specificity:

    GDF15 Polyclonal Antibody detects endogenous levels of GDF15 protein.


    Product images
    GDF15 Rabbit Polyclonal Antibody - 1 Fig
    : Western blot analysis of GDF15 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA68343, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HepG2 cell
    Lane 2: SH-SY5Y cell
    Lane 3: AGS cell
    Lane 4: CAKI-1 cell
    Lane 5: Mouse kidney
    Predicted molecular weight:34 kDa
    Observed molecular weight:34 kDa
    GDF15 Rabbit Polyclonal Antibody - 2 Fig : Western blot analysis of GDF15 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA68343, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HT1080 cell
    Lane 2: TT cell
    Predicted molecular weight:34 kDa
    Observed molecular weight:34 kDa

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