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DLAT Rabbit Polyclonal Antibody

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货号:
AWA61502
应用:
WB,IHC-P,IF-C
反应性:
Human,Mouse,Rat,Monkey
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human, Mouse, Rat, Monkey

    Molecular Wt:

    Predicted MW: 69 kDa
    Observed MW: 69 kDa

     

    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1.066mg/ml

     

    Other Names:

    E2; E2 component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; M2 Antigen Complex 70kD Subunit; mitochondrial; PBC; PDC E2; PDC-E2; PDCE2; Pyruvate dehydrogenase complex component E2; Pyruvate dehydrogenase complex E2 subunit; S acetyltransferase component of pyruvate dehydrogenase complex; DLTA; DLAT

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:5000
    IHC-P 1:100-1:1000
    IF-C 1:100-1:800

    Immunogen
    Information

    Gene Name:

    DLAT

    Protein Name:

    Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

     

    Gene ID:

    1737 (Human)  
    235339 (Mouse)  
    81654 (Rat)

    SwissPro:

    P10515 (Human)  
    Q8BMF4 (Mouse)  
    P08461 (Rat)

    Immunogen
    Information

    Subcellular Location:

    Mitochondrion matrix.

     

    Immunogen:

    Recombinant protein within rat DLAT. AA range: 227-463.

     

    Specificity:

    DLAT Polyclonal Antibody detects endogenous levels of DLAT protein.


    Product images
    DLAT Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of DLAT on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA61502, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Jurkat cell
    Lane 2: HEK293 cell
    Lane 3: K562 cell
    Lane 4: COS7 cell
    Predicted molecular weight: 69 kDa
    Observed molecular weight: 69 kDa
    Exposure time:45s
    DLAT Rabbit Polyclonal Antibody - 2 Fig : Western blot analysis of DLAT on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA61502, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: MCF-7 cell
    Lane 2: SK-BR-3 cell
    Lane 3: Daudi cell
    Predicted molecular weight: 69 kDa
    Observed molecular weight: 69 kDa
    Exposure time:15s

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