ZO-1 Rabbit Polyclonal Antibody_艾碧维生物官网

货号：

AWA47635

应用：

IHC-P,IF,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

195 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1.079mg/ml

Other Names:

Tight junction protein 1; Tight junction protein ZO 1; TJP1; ZO 1; ZO1; ZO-1; Zona occludens protein 1; Zonula occludens protein 1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

IHC-P 1:100-1:1000
IF 1:100-1:800
FCM 1:50-1:200

Immunogen
Information

Gene Name:

TJP1

Protein Name:

Tight junction protein 1

Gene ID:

7082 (Human)
21872 (Mouse)
292994 (Rat)

SwissPro:

Q07157 (Human)
P39447 (Mouse)
A6JBM5 (Rat)

Immunogen
Information

Subcellular Location:

Cell membrane. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Cell projection, podosome.

Immunogen:

Synthetic peptide of human ZO-1. AA range: 500-514 and 1682-1696.

Specificity:

ZO-1 Polyclonal Antibody detects endogenous levels of ZO-1 protein.

Product images
	Fig: Immunocytochemistry analysis of A431 cells labeling ZO-1 with Rabbit anti-ZO-1 antibody（AWA47635）at 1/200 dilution(Green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-ZO-1 antibody （AWA47635）at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti- ZO-1 antibody (AWA47635) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA47635) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig：Flow cytometric analysis of A431 cells labeling ZO-1. Overlay histogram showing A431 cells stained with ZO-1 (green line). The cell were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA47635, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005b) at 1/2500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

引用文献 (1)

BMC Complementary Medicine and Therapies IF:3.3

Ge-Gen-Qin-Lian decoction alleviates the symptoms of type 2 diabetes mellitus with inflammatory bowel disease via regulating the AGE-RAGE pathway

Background This study aimed to explore the mechanism of Ge-Gen-Qin-Lian decoction (GGQLD) in the alleviation of symptoms of type 2 diabetes mellitus (T2DM) with inflammatory bowel disease (IBD) by network pharmacology and experimental validation. Methods The active components and targets of GGQLD were identified from the TCMSP database. The potential therapeutic targets of T2DM and IBD were identified from the GEO database and 4 online disease target databases. The PPI network and KEGG/GO analyses were performed with the common targets among GGQLD, T2DM and IBD. Molecular docking was carried out between the core compounds and hub targets. To verify the above results, UHPLC-MS technology was used to identify the chemical compounds in GGQLD, and a T2DM with IBD rat model was used to explore the mechanism by which GGQLD treats T2DM with IBD. Results Totally, 70 potential therapeutic targets were identified among GGQLD, T2DM and IBD. Ten hub genes were selected from the PPI network. KEGG analysis revealed that GGQLD is tightly involved in the AGE-RAGE signaling pathway. Berberine, baicalein, wogonin, and quercitrin are the main active compounds of GGQLD. Animal experiments showed that GGQLD could decrease blood glucose and alleviate intestinal inflammation. Notably, the concentrations of AGEs, the expression of RAGE, c-JUN and NF-κB and the expression of inflammatory cytokines were decreased by GGQLD. Conclusions Our study initially demonstrated that GGQLD has favorable anti-hyperglycemic and anti-intestinal inflammation effects in a T2DM with IBD rat model, and the AGE-RAGE pathway plays a vital role in this process.

pubTime 2024-06-10

Application

Specie

Rat

Dilution