MMP13 Recombinant Rabbit Monoclonal Antibody [47F04]

货号：

AWA22037

应用：

WB,IHC-P,IF-C,IP

反应性：

Mouse

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Mouse

Molecular Wt:

54 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.058mg/ml

Other Names:

CLG3; MDST; MANDP1; Collagenase 3; Matrix metalloproteinase-13; Matrix metalloproteinase 13; MMP-13; MMP 13; MMP13

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:800
IP 0.5-4.0 ug for 0.5-3.0 mg of total protein lysate.

Immunogen
Information

Gene Name:

MMP13

Protein Name:

Collagenase 3

Gene ID:

17386 (Mouse)

SwissPro:

P33435 (Mouse)

Immunogen
Information

Subcellular Location:

Secreted, extracellular space, extracellular matrix. Secreted.

Immunogen:

Recombinant protein within Mouse MMP13.

Specificity:

MMP13 Monoclonal Antibody detects endogenous levels of MMP13 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mosue-bone tissue with Rabbit anti- MMP13 antibody （AWA22037） at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer （pH 6.0） for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody （AWA22037） at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system（ABIOWELL, AWI0629）. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Western blot analysis of MMP13 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22037, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepA1-6 cell Predicted molecular weight:54 kDa Observed molecular weight:54 kDa