STAT3 Recombinant Rabbit Monoclonal Antibody

货号：

AWA12672

应用：

WB,IHC-P,IF-C,IF-P,IP,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 88 kDa
Observed MW: 88 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.117mg/ml

Other Names:

STAT3; STAT 3; APRF; HIES; ADMIO; ADMIO1; T3; DNA binding protein APRF; Signal transducer and activator of transcription 3; Acute-phase response factor

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:800
IF-P 1:100-1:1000
IP 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate.
FCM 1:50-1:200

Immunogen
Information

Gene Name:

STAT3

Protein Name:

Signal transducer and activator of transcription 3

Gene ID:

6774 (Human)
20848 (Mouse)
25125 (Rat)

SwissPro:

P40763 (Human)
P42227 (Mouse)
P52631 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm. Nucleus.

Immunogen:

Synthetic peptide within C-terminal human STAT3.

Specificity:

STAT3 Monoclonal Antibody detects endogenous levels of STAT3 protein.

Product images
	Fig : Western blot analysis of STAT3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12672, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC12 cell Lane 2: K562 cell Lane 3: Jurkat cell Lane 4: Mouse brain Predicted molecular weight:88 kDa Observed molecular weight:88 kDa

	Fig : Western blot analysis of STAT3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12672, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: SK-BR-3 cell Predicted molecular weight:88 kDa Observed molecular weight:88 kDa

	Fig : Western blot analysis of STAT3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12672, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell Lane 2: PANC-1 cell Lane 3: Raw264.7 cell Lane 4: Rat brain Predicted molecular weight:88 kDa Observed molecular weight:88 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-STAT3 antibody (AWA12672) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12672) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Fluorescence immunohistochemical analysis of Mouse-liver tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-STAT3 antibody (AWA12672) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12672) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Immunocytochemistry analysis of Hela cells labeling STAT3 with Rabbit anti-STAT3 antibody (AWA12672) at 1/100 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-STAT3 antibody (AWA12672) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig：Immunoprecipitation of STAT3 from Hela cells was performed using STAT3 Rabbit mAb (AWA12672,1:250). Rabbit lgG isotype control was used to precipitate the Control lgG sample. The IP sample was eluted with Glycine buffer. Western blot analysis of immunoprecipitates was conducted using STAT3 Rabbit mAb (AWA12672) at a dilution of 1:1000. Goat Anti-Rabbit IgG(H+L) - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution.

	Fig: Immunocytochemistry analysis of NIH3T3 cells labeling STAT3 with Rabbit anti-STAT3 antibody (AWA12672) at 1/300 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-STAT3 antibody (AWA12672) at 1/300 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig: Fluorescence immunohistochemical analysis of Rat-Brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-STAT3 antibody (AWA12672) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12672) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

引用文献 (1)

Molecular & Cellular Toxicology IF:1.4

STAT3/NAV2 signaling promotes the progression of pulmonary fibrosis

Background Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive disorder with poor prognosis. The mechanism of IPF is poorly understood, and hence effective treatments are lacking. Neuron navigator 2 (NAV2) plays a critical role in cell migration and a variety of autoimmune diseases. However, its role in IPF is still unclear. Methods Serum samples from IPF patients and healthy volunteers were collected and NAV2 was detected via ELISAs. Bleomycin (BLM) was used to establish a pulmonary fibrosis model in C57BL/6 J mice. Adeno-associated virus (AAV6)-shRNA was used to knockdown NAV2. Serum and alveolar lavage fluid were collected and IL-6 and TNF-α were detected. The degree of alveolitis and pulmonary fibrosis were scored. NAV2, anti-alpha smooth muscle (α-SMA), and fibronectin were measured by immunohistochemistry and Western blotting. SiRNA-NAV2-treated and STAT3-inhibited MRC-5 cells were treated with transforming growth factor-β1 (TGF-β1). The expression of fibronectin, α-SMA STAT3, and NAV2 was determined by Western blotting. Results NAV2 levels were elevated in the IPF patients compared with the controls ( p < 0.05). NAV2 expression was elevated in the lung tissue of the BLM-treated mice compared with that in the control mice ( p < 0.05). When the mice were administered AAV6-shRNA-NAV2, fibrosis was alleviated and both fibronectin and α-SMA levels were reduced. TGF-β1 treatment increased the expression of STAT3 and NAV2 in MRC-5 cells. When the cells were transfected with siRNA-NAV2 or treated with a STAT3 inhibitor, fibronectin and α-SMA expression was reduced. Conclusion NAV2 signaling promotes the progression of pulmonary fibrosis. The STAT3/NAV2 axis has an important regulatory role in the TGF-β1-related epithelial–mesenchymal transition, which might provide a potential target for IPF treatment.

pubTime 2025-05-04

Application

Specie

Human

Dilution