JNK1/2/3 Recombinant Rabbit Monoclonal Antibody

货号：

AWA12669

应用：

WB,IHC-P

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 48/53 kDa
Observed MW: 48/53 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.164mg/ml

Other Names:

C Jun kinase 2; c Jun N terminal kinase 1; c Jun N terminal kinase 2; c Jun N terminal kinase 3; c-Jun N-terminal kinase 1; JNK 46; JNK-46; JNK 55; JNK; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; JNK3 alpha protein kinase; JNK3; JNK3A; Jun kinase; JUN N terminal kinase; MAP kinase 10; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 10; MAPK 8; MAPK 9; MAPK10; mapk8; MAPK9; p493F12; p54a; p54aSAPK; p54bSAPK; PRKM10; PRKM8; PRKM9; SAPK; SAPK(beta); SAPK1; SAPK1a; SAPK1b; SAPK1c; Stress activated protein kinase JNK1; Stress activated protein kinase JNK2; Stress activated protein kinase JNK3; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; JNK1/2/3

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000

Immunogen
Information

Gene Name:

MAPK8/MAPK9/MAPK10

Protein Name:

Mitogen-activated protein kinase 8/Mitogen-activated protein kinase 9/Mitogen-activated protein kinase 10

Gene ID:

5599/5601/5602 (Human)
26419/26420/26414 (Mouse)
116554/50658/25272 (Rat)

SwissPro:

P45983/P45984/P53779 (Human)
Q91Y86/Q9WTU6/Q61831 (Mouse)
P49185/P49186/P49187 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm. Nucleus. Synapse. Membrane, Mitochondrion.

Immunogen:

Recombinant protein within human JNK1. AA range: 1-423.

Specificity:

JNK1/2/3 Monoclonal Antibody detects endogenous levels of JNK1/2/3 protein.

Product images
	Fig : Western blot analysis of JNK1/2/3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA12669, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: K562 Lane 4: MCF-7 Lane 5: RAW264.7 Lane 6: PC12 Lane 7: NIH/3T3 Predicted molecular weight:48/53 kDa Observed molecular weight:48/53 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Human-colonia cancer tissue with Rabbit anti- JNK1/2/3 antibody (AWA12669) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12669) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.