SIRT3 Recombinant Rabbit Monoclonal Antibody [42A06]

货号：

AWA10553

应用：

WB,IHC-P,IF-C,IF-P,IP,ChIP,FCM

反应性：

Human,Mouse,Rat,Rabbit

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Rabbit

Molecular Wt:

Predicted MW: 44 kDa
Observed MW: 44/28 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.086mg/ml

Other Names:

hSIRT3; SIR2 like protein 3; SIR2L3; SIR2 like protein 3; Regulatory protein SIR2 homolog 3; NAD-dependent protein deacetylase sirtuin-3; mitochondrial; SIRT3

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:800
IF-P 1:100-1:1000
IP 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate.
ChIP Use 0.5~2 μg for 25 μg of chromatin
FCM 1:50-1:200

Immunogen
Information

Gene Name:

SIRT3

Protein Name:

NAD-dependent protein deacetylase sirtuin-3, mitochondrial

Gene ID:

23410 (Human)
64384 (Mouse)
293615 (Rat)

SwissPro:

Q9NTG7 (Human)
Q8R104 (Mouse)
F7EYD5 (Rat)

Subcellular Location:

Mitochondrion matrix.

Immunogen:

Recombinant protein within human SIRT3. AA range: 51-399.

Specificity:

SIRT3 Monoclonal Antibody detects endogenous levels of SIRT3 protein.

Product images
	Fig : Western blot analysis of SIRT3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10553, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse liver Lane 2: Rat liver Lane 3: Rat heart Lane 4: Mouse kidney Lane 5: Rabbit kidney Predicted molecular weight:44 kDa Observed molecular weight:44 kDa/28KDa

	Fig : Western blot analysis of SIRT3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10553, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse heart Lane 2: Mouse kidney Lane 3: Mouse liver Lane 4: A549 cell Lane 5: SIHa cell Predicted molecular weight:44 kDa Observed molecular weight:28 kDa（Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.）

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-SIRT3 antibody (AWA10553) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10553) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Immunocytochemistry analysis of Hepg2 cells labeling SIRT3 with Rabbit anti-SIRT3 antibody (AWA10553) at 1/200 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-SIRT3 antibody (AWA10553) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig: Immunocytochemistry analysis of NIH3T3 cells labeling SIRT3 with Rabbit anti-SIRT3 antibody（AWA10553）at 1/300 dilution（Green）. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-SIRT3 antibody （AWA10553） at 1/300 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L （iFluor™ 488, AWS0005） was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI（AWC0291）.