IL-1β Recombinant Rabbit Monoclonal Antibody

货号：

AWA10288

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat,Pig

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human , Mouse , Rat , Pig

Molecular Wt:

Predicted MW: 31 kDa
Observed MW: 17 kDa, 31 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

Il-1b; IL-1beta; IL1β; IL-1β

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:500
IF-C 1:50-1:500
IF-T 1:100-1:500
FCM 1:50-1:200

Immunogen
Information

Gene Name:

IL1B

Protein Name:

Interleukin-1 beta

Gene ID:

3553 (Human)
16176 (Mouse)

SwissPro:

P01584 (Human)
P10749 (Mouse)
Q63264 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm, cytosol. Secreted. Lysosome. Secreted, extracellular exosome.

Immunogen:

Synthetic peptide of human IL-1β. AA range: 206-220.

Specificity:

IL-1β Monoclonal Antibody detects endogenous levels of IL-1β protein.

Product images
	Fig : Western blot analysis of IL-1β on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10288, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell Lane 2: THP-1 cell(THP-1 treated with 80 nM TPA overnight and then 100 ng/ml LPS for 6 h and 300 ng/ml Brefeldin A for the last 3 h) Predicted molecular weight:31 kDa Observed molecular weight:17 kDa,31 kDa

	Western blot analysis of IL-1β on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10288, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell Lane 2: THP-1 THP-1 cell(THP-1 treated with 80 nM TPA overnight and then 100 ng/ml LPS for 6 h and 300 ng/ml Brefeldin A for the last 3 h) Lane 3: Raw264.7 cell Lane 4: Raw264.7 cell(treated with 100 ng/ml LPS for 7 hours and 300 ng/ml Brefeldin A for the last 3 hours) Lane 5: J774A.1 cell Lane 6: 3D4/21 cell Lane 7: A549 cell Lane 8: LLC cell Lane 9: A431 cell Lane 10: Rat brain Predicted molecular weight:31 kDa Observed molecular weight:17kDa,31 kDa

	Fig: Immunocytochemistry analysis of THP-1 cells (treated with 80 nM TPA overnight and then 100 ng/ml LPS for 6 h and 300 ng/ml Brefeldin A for the last 3 h) labeling IL-1β with Rabbit anti-IL-1β antibody (AWA10288) at 1/50 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-IL-1β antibody (AWA10288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig: Immunocytochemistry analysis of RAW 264.7 cells (100 ng/ml LPS 6h+300 ng/ml Brefeldin A 3h) labeling IL-1β with Rabbit anti-IL-1βantibody (AWA10288) at 1/50 dilution(Green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-IL-1β antibody (AWA10288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Overlay histogram showing THP-1 cells stained with IL-1β (green line). The cells were treated with 80nM TPA for 16 hours,then 100ng/mL LPS fpr 3 hours and 300ng/mL BFA for anther 3 hours,then fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA10288, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005) at 1/500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with rabbit anti-IL-1β antibody (AWA10288) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10288) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Rabbit anti-IL-1β antibody (AWA10288) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA22006) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Fluorescence immunohistochemical analysis of Mouse-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-IL-1β antibody (AWA10288) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10288) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Fluorescence immunohistochemical analysis of Rat-testicle tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-IL-1β antibody (AWA10288) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10288) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Immunocytochemistry analysis of Raw264.7 cells labeling IL-1β with Rabbit anti- IL-1β antibody (AWA10288) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti- IL-1β antibody (AWA10288) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).