JNK1/2/3(Phospho T183/T183/T221) Recombinant Rabbit Monoclonal Antibody

货号：

AWA10157

应用：

WB,IHC-P,IHC-F,IF-C,IF-T,IP,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 48/53 kDa
Observed MW: 48/53 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.075mg/ml

Other Names:

C Jun kinase 2; c Jun N terminal kinase 1; c Jun N terminal kinase 2; c Jun N terminal kinase 3; c-Jun N-terminal kinase 1; JNK 46; JNK-46; JNK 55; JNK; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2A; JNK2ALPHA; JNK2B; JNK2BETA; JNK3 alpha protein kinase; JNK3; JNK3A; Jun kinase; JUN N terminal kinase; MAP kinase 10; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 10; MAPK 8; MAPK 9; MAPK10; mapk8; MAPK9; p493F12; p54a; p54aSAPK; p54bSAPK; PRKM10; PRKM8; PRKM9; SAPK; SAPK(beta); SAPK1; SAPK1a; SAPK1b; SAPK1c; Stress activated protein kinase JNK1; Stress activated protein kinase JNK2; Stress activated protein kinase JNK3; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; JNK1/2/3; JNK1/2/3(Phospho T183/T183/T221)

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IHC-F 1:100-1:500
IF-C 1:50-1:500
IF-T 1:100-1:500
IP 1:100-1:300
FCM 1:50-1:200

Immunogen
Information

Gene Name:

MAPK8/MAPK9/MAPK10

Protein Name:

Mitogen-activated protein kinase 8/Mitogen-activated protein kinase 9/Mitogen-activated protein kinase 10

Gene ID:

5599/5601/5602 (Human)
26419/26420/26414 (Mouse)
116554/50658/25272 (Rat)

SwissPro:

P45983/P45984/P53779 (Human)
Q91Y86/Q9WTU6/Q61831 (Mouse)
P49185/P49186/P49187 (Rat)

Immunogen
Information

Subcellular Location:

Cytoplasm. Nucleus. Synapse. Membrane, Mitochondrion.

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Thr183 of human JNK1. AA range: 161-204.

Specificity:

Phospho JNK1/2/3 (T183/T183/T221) Monoclonal Antibody detects endogenous levels of Phospho JNK1/2/3 (T183/T183/T221) protein.

Product images
	Fig : Western blot analysis of JNK123(Phospho T183/T183/T221) on HEK-293 lysates.Proteins were transferred to a NC membrane and blocked with 5% BSA in TBST for 1 hour at room temperature. The primary antibody (AWA10157, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK-293 lysates Lane 2: HEK-293 lysates,Then the membrane was incubated with alkaline phosphatase Predicted molecular weight: 48,53 KDa Observed molecular weight: 48,53 KDa

	Fig: Immunocytochemistry analysis of A431 cells labeling JNK1/2/3(Phospho T183/T183/T221) with Rabbit anti-JNK1/2/3(Phospho T183/T183/T221) antibody (AWA10157) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-JNK1/2/3(Phospho T183/T183/T221) antibody (AWA10157) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Western blot analysis of JNK1/2/3 and JNK1/2/3(Phospho T183/T183/T221) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( JNK1/2/3 AWA12669, JNK1/2/3(Phospho T183/T183/T221) AWA10157 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: JNK1/2/3 AWA12669 against HEK293 cell Lane 2: JNK1/2/3(Phospho T183/T183/T221) AWA10157 aginst HEK293 cell Lane 3: JNK1/2/3 AWA12669 against HEK293 cell treated with UV (1 hour) Lane 4: JNK1/2/3(Phospho T183/T183/T221) AWA10157 aginst HEK293 cell treated with UV (1 hour) Lane 5: β-actin AWA80001 against HEK293 cell Lane 6: β-actin AWA80001 against HEK293 cell treated with UV (1 hour) Predicted molecular weight: 48,53 kDa Observed molecular weight: 48,53 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-JNK1/2/3(Phospho T183/T183/T221) (AWA10157) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10157) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Fluorescence immunohistochemical analysis of Mouse-duodenum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-JNK1/2/3(Phospho T183/T183/T221) antibody (AWA10157) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10157) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-JNK1/2/3(Phospho T183/T183/T221) antibody (AWA10157) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10157) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-liver tissue with Rabbit anti-JNK1/2/3(Phospho T183/T183/T221) antibody (AWA10157) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10157) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-JNK1/2/3(Phospho T183/T183/T221) (AWA10157) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10157) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-myocardium tissue with Rabbit anti-JNK1/2/3(Phospho T183/T183/T221) (AWA10157) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10157) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti- JNK1/2/3(Phospho T183/T183/T221) antibody (AWA10157) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10157) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig：Flow cytometric analysis of HELA cells labeling JNK1/2/3(Phospho T183/T183/T221). Overlay histogram showing HELA cells stained withJNK1/2/3(Phospho T183/T183/T221) (green line). The cell were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA10157, 1:50 ) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005b) at 1/500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).