FDX1 Recombinant Rabbit Monoclonal Antibody

货号：

AWA10150

应用：

WB,IHC-P,IF-C,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 19 kDa
Observed MW: 15 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1.193mg/ml

Other Names:

ADX; FDX; Ferredoxin-1; ferredoxin 1; LOH11CR1D; FDX1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:800
IP 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate.

Immunogen
Information

Gene Name:

FDX1

Protein Name:

Adrenodoxin, mitochondrial

Gene ID:

2230 (Human)
14148 (Mouse)
29189 (Rat)

SwissPro:

P10109 (Human)
P46656 (Mouse)
P24483 (Rat)

Immunogen
Information

Subcellular Location:

Mitochondrion matrix.

Immunogen:

Synthetic peptide within human FDX1. AA range: 51-100.

Specificity:

FDX1 Monoclonal Antibody detects endogenous levels of FDX1 protein.

Product images
	Fig :Western blot analysis of FDX1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10150, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:* Lane 1: HepG2 cell Lane 2: HEK293 cell Predicted molecular weight: 19kDa Observed molecular weight: 15kDa

	Fig :Western blot analysis of FDX1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10150, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:* Lane 1: HepA1-6 cell Predicted molecular weight: 19kDa Observed molecular weight: 15kDa

	Fig: Immunocytochemistry analysis of A549 cells labeling FDX1 with rabbit anti-FDX1 antibody (AWA10150) at 1/100 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-FDX1 antibody (AWA10150) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-RABBIT IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig：Immunoprecipitation of FDX1 from HepG2 cells was performed using FDX1 Rabbit mAb (AWA10150,1:250). Rabbit IgG isotype control was used to precipitate the Control IgG sample. The IP sample was eluted with Glycine buffer. Western blot analysis of immunoprecipitates was conducted using FDX1 Rabbit mAb (AWA10150) at a dilution of 1:1000. Goat Anti-Rabbit IgG(H+L) - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution.*

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-FDX1 antibody (AWA10150) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10150) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Immunocytochemistry analysis of HELA cells labeling FDX1 with Rabbit anti-FDX1 antibody (AWA10150) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-FDX1 antibody (AWA10150) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig :Western blot analysis of FDX1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10150, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:* Lane 1: Mouse liver Predicted molecular weight: 19 kDa Observed molecular weight: 15 kDa

	Fig :Western blot analysis of FDX1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10150, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:* Lane 1: Rat liver Predicted molecular weight: 19kDa Observed molecular weight: 15kDa

	Fig :Western blot analysis of FDX1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10150, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:* Lane 1: SiHa cell Predicted molecular weight: 19kDa Observed molecular weight: 15kDa