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O-Linked N-Acetylglucosamine Recombinant Mouse Monoclonal Antibody

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货号:
AWA06068
应用:
WB,IF-C
反应性:
Human,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human, Rat

     

    Clonality:

    Monoclonal

    Isotype:

    IgG2a

    Concentration:

    1.055mg/ml

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:10000
    IF-C 1:100-1:800

    Immunogen
    Information

    Immunogen:

    Peptide.

     

    Specificity:

    O-Linked N-Acetylglucosamine Monoclonal Antibody detects endogenous levels of O-Linked N-Acetylglucosamine protein.


    Product images
    O-Linked N-Acetylglucosamine Recombinant Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of O-Linked N-Acetylglucosamine on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06068, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Jurkat cell
    Lane 2: SH-SY5Y cell
    Lane 3: Hela cell
    Lane 4: A549 cell
    Lane 5: RBL-2H3 cell
    Lane 6: MCF-7 cell
    Lane 7: SHZ-88 cell
    Observed molecular weight:>45 kDa

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