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RUNX2 Recombinant Mouse Monoclonal Antibody

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货号:
AWA06067
应用:
WB,IHC-P,IF-C,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human, Mouse, Rat

    Molecular Wt:

    57 kDa

     

    Clonality:

    Monoclonal

    Isotype:

    IgG2a

    Concentration:

    0.854mg/ml

     

    Other Names:

    OSF2; OSF 2; OSF-2; Core-binding factor subunit alpha-1; CBF-alpha-1; AML3; AML-3; CCD; CCD1; CLCD; CBFA1; PEA2aA; PEBP2aA; RUNX2

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:5000
    IHC-P 1:100-1:1000
    IF-C 1:100-1:800
    FCM 1:50-1:200

    Immunogen
    Information

    Gene Name:

    RUNX2

    Protein Name:

    Runt-related transcription factor 2

     

    Gene ID:

    860 (Human)    
    12393 (Mouse)

    SwissPro:

    Q13950 (Human)    
    Q08775 (Mouse)    
    Q9Z2J9 (Rat)

    Immunogen
    Information

    Subcellular Location:

    Nucleus. Cytoplasm.

     

    Immunogen:

    Recombinant protein within human RUNX2.

     

    Specificity:

    RUNX2 Monoclonal Antibody detects endogenous levels of RUNX2 protein.


    Product images
    RUNX2 Recombinant Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of RUNX2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06067, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: U251 cell
    Lane 2: MCF-7 cell
    Lane 3: ATDC5 cell
    Lane 4: COS7 cell
    Predicted molecular weight:57 kDa
    Observed molecular weight:57 kDa

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