Occludin Recombinant Mouse Monoclonal Antibody

货号：

AWA06066

应用：

WB,IHC-P,IHC-F,IF-C,IP,FCM

反应性：

Human,Mouse,Rat,Dog

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat, Dog

Molecular Wt:

59 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

0.843mg/ml

Other Names:

Occludin-1; Occludin1; Occludin 1; OCLN; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Tight junction protein occludin; Occludin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IHC-F 1:100-1:1000
IF-C 1:100-1:800
IP 0.5-4.0 ug for 0.5-3.0 mg of total protein lysate.
FCM 1:50-1:200

Immunogen
Information

Gene Name:

OCLN

Protein Name:

Occludin

Gene ID:

100506658 (Human)
18260 (Mouse) 83497 (Rat)

SwissPro:

Q16625 (Human)
Q61146 (Mouse)
Q6P6T5 (Rat)

Immunogen
Information

Subcellular Location:

Cell membrane. Cell junction, tight junction.

Immunogen:

Recombinant protein within human Occludin.

Specificity:

Occludin Monoclonal Antibody detects endogenous levels of Occludin protein.

Product images
	Fig : Western blot analysis of Occludin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06066, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HacaT cell Lane 2: HC11 cell Lane 3: GES-1 cell Lane 4: Mouse brain Lane 5: Rat brain Lane 6: A431 cell Predicted molecular weight:59 kDa Observed molecular weight:59 kDa

	Fig : Western blot analysis of Occludin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06066, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: CACO-2 cell Lane 2: DLD-1 cell Lane 3: AGS cell Lane 4: SK-N-MC cell Lane 5: ACHN cell Predicted molecular weight:59 kDa Observed molecular weight:59 kDa