GANAB Recombinant Mouse Monoclonal Antibody [M48A03]

货号：

AWA06049

应用：

WB,IHC-P,IF-P,IP

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

107 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

0.591mg/ml

Other Names:

G2AN; KIAA0088; Neutral alpha-glucosidase AB; Neutral alpha glucosidase AB; Alpha-glucosidase 2; Glucosidase II subunit alphak; Glucosidase II alpha; Glu II; GluII; GANAB

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000

IHC-P 1:100-1:1000

IF-P 1:100-1:1000

IP 0.5-4.0 ug for 0.5-3.0 mg of total protein lysate.

Immunogen
Information

Gene Name:

GANAB

Protein Name:

Neutral alpha-glucosidase AB

Gene ID:

23193 (Human)

14376 (Mouse)

293721 (Rat)

SwissPro:

Q14697 (Human)

Q8BHN3 (Mouse)

D3ZAN3 (Rat)

Immunogen
Information

Subcellular Location:

Endoplasmic reticulum. Golgi apparatus. Melanosome.

Immunogen:

Recombinant protein within human GANAB. AA range: 29-450.

Specificity:

GANAB Monoclonal Antibody detects endogenous levels of GANAB protein.

Product images
	Fig : Western blot analysis of GANAB on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06049, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: C6 cell Lane 2: EL-4-B5 cell Lane 3: MCF-7 cell Lane 4: SH-SY5Y cell Lane 5: 3D4/21 cell Lane 6: Mouse testis Lane 7: U87-mg cell Lane 8: Mouse brain Lane 9: Rat brian Predicted molecular weight:107 kDa Observed molecular weight:107 kDa

	Fig : Western blot analysis of GANAB on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06049, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1cell Lane 2: A431 cell Predicted molecular weight:107 kDa Observed molecular weight:107 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-epididymis tissue with Mouse anti-GANAB antibody (AWA06049) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06049) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig：Immunoprecipitation of GANAB from MCF-7 cells was performed using GANAB Mouse mAb (AWA06049, 1 ug antibody for 2 mg of total protein lysate). Mouse IgG isotype control was used to precipitate the Control IgG sample. The IP sample was eluted with Glycine buffer. Western blot analysis of immunoprecipitates was conducted using GANAB Rabbit mAb (AWA10266) at a dilution of 1:1000. Goat Anti-Rabbit IgG(H+L) - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution.