CD16/32 Recombinant Mouse Monoclonal Antibody [40C2]

货号：

AWA06009

应用：

WB,FCM

反应性：

Mouse

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Mouse

Molecular Wt:

Predicted MW: 35 kDa
Observed MW: 48 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.024mg/ml

Other Names:

CD16a; CD16b; CD32; CD32A; CD32B; CDw32; Fc fragment of IgG low affinity IIa receptor; Fc fragment of IgG low affinity IIb receptor; Fc fragment of IgG low affinity IIIa recepto; Fc fragment of IgG low affinity IIIb receptor; Fc gamma receptor III A; Fc gamma receptor IIIb; Fc gamma RIIa; Fc gamma RIIb; Fc gamma RIII beta; Fc-gamma RII-a; Fc-gamma-RIIa antibody FCG2A; FcGR; FCGR2A; FCGR2B; FCGR3; FCGR3A; FCGR3B; FcRII-a; FcRIII; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
FCM 1:50-1:200

Immunogen
Information

Gene Name:

Fcgr2/Fcgr3

Protein Name:

Low affinity immunoglobulin gamma Fc region receptor II/ Low affinity immunoglobulin gamma Fc region receptor III

Gene ID:

14130 (Mouse)

SwissPro:

P08101/P08508 (Mouse)

Subcellular Location:

Cell membrane. Cytoplasm, cytoskeleton. Secreted.

Immunogen:

Mouse BALB/c Macrophage J774.

Specificity:

CD16/32 Monoclonal Antibody detects endogenous levels of CD16/32 protein.

Product images
	Fig：Flow cytometric analysis of J774A.1 cells labeling CD16/32. Overlay histogram showing J744A.1 cells stained with CD16/32 (green line). The cell were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA06009, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody (AWS0003a) at 1/3000 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

	Fig : Western blot analysis of CD16/32 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06009, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis Predicted molecular weight:35 kDa Observed molecular weight:48 kDa