ATM(Phospho Ser1981) Recombinant Mouse Monoclonal Antibody [46C04]

货号：

AWA02885

应用：

WB,IHC-P,IF-C,IF-P,IP

反应性：

Human

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human

Molecular Wt:

351 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1mg/ml

Other Names:

ATM; Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated; ATM(Phospho Ser1981)

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:800
IF-P 1:100-1:1000
IP 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate.

Immunogen
Information

Gene Name:

ATM

Protein Name:

Serine-protein kinase ATM

Gene ID:

47 2 (Human)

SwissPro:

Q1331 5 (Human)

Immunogen
Information

Subcellular Location:

Nucleus. Cytoplasmic vesicle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Peroxisome matrix.

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Ser1981 of human ATM. AA range: 1940-1989.

Specificity:

Phospho ATM (Ser1981) Monoclonal Antibody detects endogenous levels of Phospho ATM (Ser1981) protein.

Product images
	Fig : Western blot analysis of ATM(Phospho Ser1981) on Hela lysates.Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA02885, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela lysates Lane 2: Hela lysates, The NC membrane with this cell lysate was treated with alkaline phosphatase. Predicted molecular weight: 351 KDa Observed molecular weight: 351 KDa

	Fig : Western blot analysis of ATM(Phospho Ser1981) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA02885, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: Ramos cell Lane 3: HepG2 cell Predicted molecular weight:351 kDa Observed molecular weight:351 kDa