HA Tag Recombinant Mouse Monoclonal Antibody

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货号：

AWA06021

应用：

WB,IF,IP,ChIP

反应性：

Speciesindependent

来源：

Mouse

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Species independent

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.071mg/ml

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IF 1:100-1:800
IP 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate.
ChIP Use 0.5~2 μg for 25 μg of chromatin

Immunogen
Information

Gene Name:

HA-Tag

Protein Name:

HA-Tag

Immunogen
Information

Immunogen:

Synthetic peptide with HA-tag.Derived from the human influenza hemaglutinin protein.

Specificity:

The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells.

Product images
	Fig : Western blot analysis of HA Tag on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06021, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293F cell Lane 2: 293F transfected with HA-tag cell bserved molecular weight: 37kda Exposure time：15s

	Fig：Immunoprecipitation of recombinant HA tag protein from HEK293F cell was performed using HA Tag Recombinant Mouse Monoclonal Antibody (AWA06021,1:250). Mouse lgG isotype control was used to precipitate the Control lgG sample. The IP sample was eluted with Glycine buffer. Western blot analysis of immunoprecipitates was conducted using HA Tag Recombinant Mouse Monoclonal Antibody (AWA06021) at a dilution of 1:3000. Goat Anti-Mouse IgG(H+L) - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution.