Myc tag Recombinant Mouse Monoclonal Antibody

货号：

AWA06017

应用：

WB,IF,IP

反应性：

Speciesindependent

来源：

Mouse

规格 价格 库存
50μL ¥550 1-3个工作日
100μL ¥900 1-3个工作日
200μL ¥1500 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Species independent

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.054mg/ml

Other Names:

Myc tag; Myc Epitope Tag

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IF 1:100-1:800
IP 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate.

Immunogen
Information

Gene Name:

Myc tag

Protein Name:

Myc tag

Immunogen
Information

Immunogen:

Synthesized peptide of human Myc tag. AA range: 23-37.

Specificity:

Myc tag Monoclonal Antibody detects endogenous levels of Myc tag protein.

Product images
	Fig : Western blot analysis of Myc tag on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06017, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HE293F cell Lane 2: HE293F transfected with c-myc-tag cell Observed molecular weight:90~100 kDa

	Fig：Immunoprecipitation of recombinant c-myc tag protein from HEK293F cell was performed using c-myc tag Recombinant Mouse Monoclonal Antibody (AWA06017,1:250). Mouse lgG isotype control was used to precipitate the Control lgG sample. The IP sample was eluted with Glycine buffer. Western blot analysis of immunoprecipitates was conducted using c-myc tag Recombinant Mouse Monoclonal Antibody (AWA06017) at a dilution of 1:3000. Goat Anti-Mouse IgG(H+L) - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution.

	Fig: Immunocytochemistry analysis of HE293F cell tranferd with c-myc tag labeling c-myc tag with Mouse anti-c-myc tag antibody (AWA06017) at 1/200 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-c-myc tag antibody (AWA06017) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).