V5 tag Recombinant Mouse Monoclonal Antibody

货号：

AWA06015

应用：

WB,IHC-P,IF-C,IP

反应性：

Speciesindependent

来源：

Mouse

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Species independent

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.023mg/ml

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:800
IP 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate.

Immunogen
Information

Gene Name:

V5-Tag

Protein Name:

V5-Tag

Immunogen
Information

Immunogen:

V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr.

Specificity:

This antibody recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

Product images
	Fig: Immunocytochemistry analysis of V5-transfected HEK293 cells labeling V5 tag with Mouse anti-V5 tag antibody (AWA06015) at 1/150 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-V5 tag antibody (AWA06015) at 1/150 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig：Immunoprecipitation of recombinant V5 tag protein from HEK293F cell was performed using V5 tag Recombinant Mouse Monoclonal Antibody (AWA06015,1:250). Mouse lgG isotype control was used to precipitate the Control lgG sample. The IP sample was eluted with Glycine buffer. Western blot analysis of immunoprecipitates was conducted using V5 tag Recombinant Mouse Monoclonal Antibody (AWA06015) at a dilution of 1:3000. Goat Anti-Mouse IgG(H+L) - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution.