GFP TAG Recombinant Mouse Monoclonal Antibody

货号：

AWA00760

应用：

WB,IHC-P,IF,IP

反应性：

Speciesindependent

来源：

Mouse

规格 价格 库存
50μL ¥550 1-3个工作日
100μL ¥900 1-3个工作日
200μL ¥1500 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Species independent

Molecular Wt:

27 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.024mg/ml

Other Names:

GFP; Green fluorescent protein; yfp; YFP; GFP tag; eGFP; eYFP; CFP; BFP; AcGFP; GFP S65T

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:500
IF 1:100-1:800
IP 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate.

Immunogen
Information

Gene Name:

GFP-Tag

Protein Name:

GFP Tag

Immunogen
Information

Subcellular Location:

Secreted.

Immunogen:

Recombinant protein with GFP.

Specificity:

GFP Monoclonal Antibody detects endogenous levels of GFP protein.

Product images
	Fig : Western blot analysis of GFP TAG on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00760,1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK293F supernatant Lane 2: HEK293F transfected with GFP-tag cell Predicted molecular weight: 27 kDa Observed molecular weight: 27 kDa Exposure time：7min

	Fig: Immunocytochemistry analysis of GFP-transfected HEK293 cells labeling GFP with Mouse anti-GFP antibody (AWA00760) at 1/150 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-GFP antibody (AWA00760) at 1/150 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig: Immunocytochemistry analysis of GFP-transfected HEK293 cells labeling GFP with Mouse anti-GFP antibody (AWA00760) at 1/300 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-GFP antibody (AWA00760) at 1/300 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).